An experimental study about the influence of bone marrow mesenchymal stem cells allotransplantation on hepatocyte proliferation in acute hepatic failure rat model

Objective To explore the mechanism of transplantation of bone marrow mesenchymal stem cells in the treatment of acute liver failure.Methods Cultured and amplified the rat bone marrow mesenchymal stem cells by adherent culture method from primary generation to fifth generation.Acute hepatic failure was induced by two injections of D-galactosamine and one injection of Lipopolysaccharide,delivered intraperitoneally with a 12-hour interval between injections.1 ml BMSCs 1.4 × 107/kg or 0.9％ NaCl solution (control) was infused into caudal vein.PCNA in liver tissue was detected by immunohistochemistry.The level of CyclinD1 mRNA in liver tissue was observed.Results BMSCs in vitro culture were homogenous population and exhibited a spindle-shaped morphology.BMSCs expressed CD29 and CD90,didn' t express CD1 lb or CD45.BMSCs therapy inhibited liver enzyme release (P ＜ 0.05).BMSCs therapy improved gross and microscopic liver histopathology (P ＜ 0.05).BMSCs therapy enhanced liver regeneration (P ＜ 0.05).BMSCs therapy up-regulated the expression of CyclinD1 mRNA (P ＜ 0.05).Conclusion BMSCs promote the regeneration of liver tissues.BMSCs transplantation has a therapy effect on acute liver failure. Key words: Bone marrow mesenchymal stem cell;  5 ethynyl-2 deoxyuridine;  Acute hepatic failure; Transplantation;  Proliferation