The applicatton value of DNA ploidy analysis in the diagnosis of pleural effusion

Objective To investigate the clinical value ofDNA ploidy analysis in the diagnosis of benign and malignant pleural effusion.Methods DNA ploidy in 24 benign pleural effusion and 39 malignant pleural effusion were detected by flow cytometry (FCM) and compared with the results of cytologic detection at the same time.Results The positive rates of FCM detection in benign and malignant pleural effusion were 8.33％(2/24) and 64.10％ (25/39),there was significant difference (P＜0.05).The positive rates of cytologic detection in benign and malignant pleural effusion were 4.17％( 1/24 ) and 53.85％( 21/39),there was significant difference (P＜0.05).The sensitivity of FCM and cytologic detection in malignant pleural effusion was 64.10％ (25/39) and 53.85％ (21/39),the specificity of two methods was 91.67％ (22/24) and 95.83％ (23/24.),the results of two methods showed no significant differences (P ＞0.05).Conclusion DNA ploidy analysis by FCM has important clinical value in the diagnosis of benign and malignant pleural effusion. Key words: Pleural effusion; Flow cytometry; DNA ploidy analysis