The role of microRNA-146b on proliferation and apoptosis of thyroid carcinoma

Objective To explore the role of microRNA (miRNA, miR)-146b on proliferation and apoptosis of thyroid carcinoma. Methods After miR-146b inhibitor and miR-146b NC were transfected into SW579 cell by liposome LipofectamineTM3000, the expression of miR-146b, cell viability, cell apoptotic rate, cell cycle, the expression of B cell lymphoma/lewkmia-2 (bcl-2), bcl-2 related X protein (bax), cyclin dependent kinases (CDK4), phosphorylation of nuclear factor kappa B (NF-κB) p65 was detected. Results The expression of miR-146b in miR-146b inhibitor group (0.38±0.05) was lower than that in miR-146b NC (1.00±0.09) (P<0.05). Cell viability in miR-146b inhibitor group (0.36±0.04) was lower than that in miR-146b NC group (0.59±0.06) (P<0.05). Cell apoptosis rate (34.58±3.50)% was higher than that in miR-146b NC group (4.28±0.43)% (P<0.05). The cell cycle in miR-146b inhibitor group (44.32±4.43)% was longer than miR-146b NC group (34.08±3.42)% (P<0.05). The expression of bcl-2, Cyclin D1 and CDK4, phosphorylation of NF-κB p65 in miR-146b inhibitor group was lower than that in miR-146b NC group (P<0.05), the expression of bax in miR-146b inhibitor group was higher than that in miR-146b NC group (P<0.05). Conclusion MiR-146b inhibitor might inhibit the proliferation of SW579 cell and induce cell apoptosis via blocking NF-κB signaling pathway. Key words: MicroRNA-146b; Thyroid carcinoma; Proliferation; Apoptosis