Anti-Interleukin-17 antibodies attenuate airway inflammation in cigarette smoke-induced chronic obstructive pulmonary disease in mice

Objective To investigate the significance of interleukin-17(IL-17)antibodies in cigarette smoke-induced chronic obstructive pulmonary disease(COPD)in mice.Methods Male C57/BL6 mice were randomly divided into COPD group(n=8),COPD+anti-IL-17 Ab group(n=8),and normal control group (n=10).The Rumher of cells in bronchoalveolar lavage fluid(BALF)was calculated and the concentrations of IL-17 in lung tissues was measured by enzyme-linked immunosorbent assay.Histoiogic analysis of the lungs was done in each group.Results The intra-airway pressure(IP),peak expiratory flow(PEF),and Ip slope in anti-IL-17 Ab group were insignificant compared with those of COPD group.The total cell count in BALF was significantly higher in COPD group than in normal control group(P＜0.01).Compared with COPD group.the total cell count in COPD+anti-IL-17 Ab group was decreased[(19.64±1.89)×104/ml and(15.47±2.99)×104/ml(P＜0.01)],neutrophils in BALF were obviously decreased,(22.98±8.46)%,(8.58±6.77)%(P＜0.01).The airway inflammation was alleviated in COPD+anti-IL-17 Ab group by histologic analysis.The concentration of IL-17 in lung tissue was significantly lower in COPD+anti-IL-17 Ab group [(0.084±0.041)pg/mg Pro] than in the COPD group[(0.221±0.081)pg/mg pro](P＜0.01).Conclusions IL-17 might play a role in airway neutrophilic inflammation in cigarette smoke-induced COPD in mice.Anti-IL-17 could decrease the Rumbet of neutrophils in the BALF and attenuate the neutrophilic airway inflammation. Key words: Chronic obstructive pulmonary disease; Disease models; Interleukin-17 antibody; Airway inflammation