Effects of rosiglitazone treatment on TGF-β1/JNK signaling pathways in otsuka long-evans tokushima fatty ratspulmonary

Objective To approach effects of rosiglitazone treatment on transforming growth factor-β1/c-Jun NH2-terminal kinases(TGF-β1/JNK) signaling pathways in otsuka long-evans tokushima fatty(OLETF) rats. Methods Experimental rats were divided into three groups: OLETF group, rosiglitazone-treated group(OLETF/L group), and long evans tokushima otsuka(LETO group) as control. The expression of TGF-β1, p-JNK, collagen type Ⅰ in pulmonary of rats were examined by immunohistochemistry. Results The expression of TGF-β1, p-JNK and mean optical density, the ratio of positive area and total area of, collagen type Ⅰ were increased in pulmonary of OLETF rats than those of LETO rats(7.4438±0.6397 vs 1.1844±0.2066,5.7025±0.5530 vs 1.5875±0.1727 and 0.1195±0.0183 vs 0.0154±0.0016,0.0322±0.0058 vs 0.0033±0.0010;0.2628±0.0279 vs 0.0831±0.0096,0.0909±0.0138 vs 0.0385±0.0082, P<0.01). The expression of TGF-β1,p-JNK and mean optical density, the ratio of positive area and total area of, collagen type Ⅰ were decreased in OLETF/L group as compared with OLETF group(1.8398±0.3591 vs 7.4438±0.6397,1.7500±0.1690 vs 5.7025±0.5530 and 0.0223±0.0053 vs 0.1195±0.0183,0.0052±0.0019 vs 0.0322±0.0058;0.1152±0.0313 vs 0.2628±0.0279,0.0535±0.0119 vs 0.0909±0.0138, P<0.01). Conclusions Rosiglitazone may improve diabetic pulmonary fibrosis probably by regulating TGF-β1/JNK signaling pathways. Key words: Diabetes mellitus;  Pulmonary;  Rosiglitazone;  Transforming growth factor-β1;  c-Jun NH2-terminal kinases