2009•中国医师杂志Requires access
The biological characteristics of adult human marrow mesenchymal stem cells cultured in umbilical cord serum
Wenyong Kuang, Ruijuan Li, Mincui Zheng
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Abstract
Objective To observe biological characteristics of adult human marrow mesenchymal stem cells(BM-MSCs)cultured by the umbilical cord serum.Methods BM-MSCs were obtained from fresh adult human bone marrow and cultured with DMEM/F12 which included 10%umbilical cord blood serum.The surface antigens of BM-MSCs were detected by flow cytometry.BM-MSCs were cultured into differentiation of osteoblasts and adipocytes with conditioned medium for osteogenesis and adipogenesis. Results The antigen of CD29.CD73,and CD105 were high expressed on BM-MSCs.The antigen of CD34,CD45 and CD31 were not expressed on BM-MSCs.The BMMSCs were differentiated into osteoblasts and adipocytes.Conclusion BM-MSCs have high purity and differentiation potency under cuhuring with umbilical cord blood serum.The umbilical cord serum is adapted to clinical application.
Key words:
Fetal blood; Cell culture techniques/MT; Hematopoietic stem cells; Bone marrow cells