2018•Chin J Cell Stem Cell (Electronic Edition)Requires access
Analysis of the effects of quercetin on the biological behavior of human glioma stem cells and the influence of miR-29s family
Dongrui Wang, Yongci Zhang
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Abstract
Objective
The effects of quercetin on the biological behavior of human glioma stem cells and the miR-29s family were investigated and analyzed.
Methods
The stem cell culture medium on U87 glioma cells were cultured to detect the inhibition rate of quercetin on the proliferation of BGSCs cells by CCK-8 method, and were detected by flow cytometry of quercetin on the apoptosis of BGSCs cells, the effect of real-time PCR on miR-29a miR-29b and the identification of quercetin and miR-29c expression in BGSCs cells.
Results
With the increase of quercetin concentration, the proliferation inhibition rate of BGSCs cells increased significantly [24 h 0 μg/ml (0.00±0.12) %, 10 μg/ml (1.36±0.38) %, 20 μg/ml (15.33±3.01) %, 40 μg/ml (29.50±4.57) %, 80 μg/ml (40.21±6.42) %, 160 μg/ml (61.21±7.48) , F = 76.273, P < 0.05; 48 h 0 μg/ml (0.09±0.05) %, 10 μg/ml (9.84±2.17) %, 20 μg/ml (28.57±3.84) %, 40 μg/ml (43.59±5.21) %, 80 μg/ ml (59.50±3.28) %, 160 μg/ml (70.21±9.48) %, F = 85.392, P < 0.05]. The inhibition rate of 48 h on BGSCs cells was significantly higher than that of 24 h (P < 0.05). With the increase of quercetin concentration, the apoptosis rate of BGSCs cells increased significantly [0 μg/ml (13.42±1.21)%, 20 μg/ml (38.47±9.28)%, 40 μg/ml (59.34±7.20)%, 80 μg/ml (71.42±9.47)%, F = 57.493, P < 0.05]. Quercetin treatment of different concentrations of BGSCs cells can promoted the relative expression of miR-29s family miR-29a/b/c in BGSCs cells. With the increase of quercetin concentration, the relative expression of miR-29s family in BGSCs cells increased significantly [miR-29a 0 μg/ml (1.04±0.08), 20 μg/ml (1.16±0.05), 40 μg/ml (1.30±0.10)、80 μg/ ml (1.41±0.09) , F = 19.281, P < 0.05; miR-29b 0 μg/ml (1.06±0.09) , 20 μg/ml (1.13±0.05) , 40 μg/ml (1.25±0.07) , 80 μg/ml (1.30±0.09) , F = 13.427, P < 0.05; miR-29c 0 μg/ml (1.03±0.07) , 20 μg/ml (1.15±0.03) , 40 μg/ml (1.22±0.06) , 80 μg/ml (1.31±0.08) , F = 14.502, P < 0.05].
Conclusion
Quercetin can effectively inhibit the proliferation of human glioma stem cells, promote the apoptosis of human glioma stem cells, and promote the expression of miR-29s family in human glioma stem cells.
Key words:
Quercetin; Human glioma stem cells; Biological behavior; MicroRNAs