Inhibitory effects of microRNA-105 on proliferation and migration of human glioma cells

Objective To investigate the microRNA-105 (miR-105) expression level in brain glioma and the role of miR-105 in the progression and metastasis of brain glioma and assess its value in predicting the prognosis of brain glioma patients. Methods Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of miR-105 gene in brain glioma and peri-cancerous tissues. The miR-105 mimic and miR-105 inhibitor were transfected to U87 cells respectively. Cell proliferation was analyzed by cell counting assay kit-8 (CCK-8). Cell invasion assays was analyzed by Transwell assay. Results Real-time PCR showed that the miR-105 expression in human brain glioma tissues was lower than that in non-cancerous brain tissues (6.043±0.427 vs. 5.216±0.418) The expression of miR-105 in brain glioma was reduced to 56.37% of that in peri-cancerous tissues (P=0.000), and was negatively associated to tumor grade (P=0.001). The results of CCK-8 proliferation assay showed that cell proliferation speed was reduced by 0.365±0.033 (50.65%, P=0.002) in miR-105 mimics-transfected cells as compared with that in the untreated cells, and after inhibiting miR-105, the speed was increased by 2.434±0.030 (54.76%, P=0.021). The invasion of the cells that were transfected with the miR-105 mimic was dramatically decreased as compared with the scrambled control and untreated cells (P=0.009). Conclusion miR-105 functions as a tumor suppressor miRNA in brain glioma. The expression of miR-105 is aberrantly low in glioma tissues. Transient transfection of miR-105 mimics leads to decreased proliferation and progression in U87 glioma cells. miR-105 plays an important role in the development of brain glioma. Key words: MicroRNA-105; Glioma; Proliferation; Migration