2016•Chin J EndemiolRequires access
The effects of sodium arsenite on mRNA expression level of pigment epithelium-derived factor and apoptosis-related factors in PC12 cells
Xiaohui Cui, Wei Zhang, Yuanyuan Li, Liyan Sun, Yanhui Gao, Lixin Jiang, Lisha Qu, Xiaona Liu, Jinhui Zhang, Chunpeng Lyu, Dianjun Sun
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Abstract
Objective
To observe the effects of different levels of sodium arsenite (NaAsO2) on mRNA expression of pigment epithelium-derived factor (PEDF) and apoptosis-related factors in PC12 cells (rat neuron properties pheochromocytoma).
Methods
PC12 cells were treated with different levels of NaAsO2 [0 (control group), 2, 5, 10 μmol/L] for 24 hours. The mRNA expression of PEDF and apoptosis-related factors (Bax, Bcl-2) were detected by real-time quantitative PCR.
Results
There were significant differences in the mRNA expressions of PEDF between the 4 groups (F = 102.28, P 0.05); there were significant differences in the mRNA expressions of Bcl-2 between the 4 groups (F = 19.87, P < 0.05), the mRNA expressions of Bcl-2 in the group of 2, 5, 10 μmol/L (0.65 ± 0.03, 0.49 ± 0.04, 0.57 ± 0.09) were lower than that of control group (0.95 ± 0.11, all P < 0.05); there were significant differences in the mRNA expressions of Bax/Bcl-2 between the 4 groups (F = 8.352, P < 0.05), the mRNA expressions of in the group of 5, 10 μmol/L (1.80 ± 0.72, 1.82 ± 0.36) were higher than that of control group (1.02 ± 0.24, all P < 0.05).
Conclusion
NaAsO2 may increase the expression of apoptosis-related factorsBax/Bcl-2 mRNA by decreasing the expression of PEDF mRNA in PC12 cells, leading to apoptosis in PC12 cells.
Key words:
Sodium arsenite; PC12 cells; Pigment epithelium-derived factor; Apoptosis-related factors