Study on the inhibition effects of Kudiezi injection to Lewis lung cancer in vitro and in vivo

Objective To observe the inhibition effects to Lewis lung cancer of kudiezi injection (KDI) in vitro and in vivo. Methods After administration of 12.5-400. 0 g/L KDI for 24-72 h, the MTT method was used to investigate the inhibitory effect of KDI on Lewis lung cancer cells. Cell apoptosis and cell cycle arrest were investigated by flow cytometry (FCM). Results KDI significantly inhibit the growth of Lewis lung cancer cells in dose-dependent manners. FCM revealed that after treatment with different dose of KDI (5,10,20 g/kg)for 14 d,the number of G0/G1 stage cell was (68. 10 ±3.43)%, (80.06 ±5.48)%,(78.36 ± 6. 68) %, and that of S stage cells was ( 30. 98 ± 1.99 ) %, ( 17. 48 ± 6. 36) %, ( 17. 05 ± 6. 35 ) %respectively, and that of apoptotic rate was 8. 96%, 17.40%, 10. 34% respectively. Conclusion KDI can inhibit Lewis lung cancer cells in vitro and in vivo,which was probably contributed to induce apoptosis and G0/G1 cell cycle arrest in Lewis lung cancer cells. Key words: Lung cancer; Kudiezi injection; Proliferation; Apoptosis; Cell cycle