Effect of gastrodin on rat vascular smooth muscle cell migration induced by platelet-derived growth factor-BB

Objective To investigate the effect of gastrodin on rat vascular smooth muscle cell （VSMC） migration induced by platelet-derived growth factor-BB （PDGF-BB） and its possible mechanisms. Methods Enzyme digestion method was used to obtain rat aortic VSMCs and be purified by passage. Immunofluorescence staining was used to identify VSMC marker proteins. A PDGF-BB induced cell migration model was established. Transwell chamber assay was used to evaluate the effect of gastrodin on PDGF-BB induced VSMC migration. Western blots were performed to detect the phosphorylation levels of c-jun N-termi- nal kinase （JNK）. Results The purity of primary cultured VSMC was more than 99%. The VSMC migrated number in the PDGF-BB group was 85.2 ± 3. 486 per field. It was significantly more than 42. 5± 1. 927 per field in the control group （t=9. 981, P〈0. 001）, and gastrodin was enable to make PDGF-BB induced the number of VSMC migration significantly reduce to 71.3 ±1. 783 per filed （t = 3. 550, P = 0. 002）. Western blots analysis showed that gastrodin inhibited PDGF-BB induced JNK phosphorylation （0. 190 ± 0. 015 vs. 0. 190± 0. 015; t = 14. 548, P =0. 000）. Conclusions Gastrodin inhibits PDGF-BB induced VSMC migration, its mechanisms may be associated with the inhibition of the JNK signaling pathway activation. Key words: Gastrodin;  Cell Movement;  Muscle, Smooth, Vascular;  Rats