Effect of over-expression of special AT-rich sequence binding protein1 gene on proliferation and invasion of human prostate carcinoma DU145 cells

Objective To observe the effects of over-expression of special AT-rich sequence binding proteinl (SATB1) gene on proliferation and invasion of human prostate carcinoma DU145 cells.Methods The pcDNA3.1-SATB1 was transfected into DU145 cells by applying LipofectamineTM 2000.The pcDNA3.1 empty vector and un-transfected cells were used as negative controls.Cells were collected and assayed at 12,24,and 36 h after transfection.The mRNA and protein expression of SATB1 was detected by using reverse transcription-polymerase chain reaction (RT-PCR),and Western blotting respectively.Cell proliferation was assayed by using cell counting kit-8 (CCK-8) method.The invasion ability was examined by Transwell assay.Results SATB1 mRNA and protein expression levels in pcDNA3.1-SATB1-transfected group were 170.30 ±2.063 and 56.3 ± 3.7 respectively,which were significantly higher than in control groups (P ＜ 0.01).Proliferation rate of DU145 cells transfected with pcDNA3.1-SATB1 for 24,48 and 72 h was (53.2 ± 5.8) ％,(62.3 ± 4.0) ％,and (76.7 ± 3.0) ％ respectively,which was significantly higher than in control groups.The Transwell assay revealed that the number of invasive cells in pcDNA3.1-SATB1 group (288.3 ± 4.5) was significantly greater than in control groups [(131.0 ± 7.9),(130.3 ±5.5)] (P ＜0.05).Conclusion Over-expression of SATB1 gene can promote proliferation and increase the invasion ability of DU145 cells. Key words: Special AT-rich sequence binding protein1 ;  Prostate carcinoma;  Invasion