2008•中国医师杂志Requires access
The change of apoptosis and chemotherapeutics resistance after multiple thermotherapy on the HepG2 cell
Chunhong Qin, Yongguo Li, Jang Wu, HE Ku-han, Shuke Fei
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Abstract
Objective To investigate the change of apoptosis and drug resistance after multiple thermotherapy On the HepG2 Cell.Methods After HepG2 cell were treaded by ten repeated cycles of exposure at 43 degree C for 80 minutes twice a day，the sensibility of HepG2 cell to Adfiamyein was analyzed by MTr assay.The apoptosis Was detected by kit，and the expression of Bcl-2 or Bax protein and mRNA were detected by immunohistochemical method and RT-PCR respective.Results The drug resistance to Adfiamycin Was gained by HepG2 cell after multiple thermotherapy.The rate of apoptosis was decreased，the expression of Bcl一2 protein and mRNA Was strengthened，and the ratio of Bcl-2/Bax increased.Conclusions The resistance of heated HepG2 cell to chemotherapeutics Was related with thedecreased rate of apoptosis，overexpression of Bcl-2 protein and mRNA，and increased ratio of Bcl-2/Bax.
Key words:
Hyperthermia,induced/MT; Liver neoplasms; Apoptosis; Drug tolerance; Genes,bcl-2