Modified in vitro sperm selection technology

Objective To improve in vitro sperm selection technology basing on WHO lab mamual for Examination and Process of Human Semen ( fifth edition ) to offer patients with a safer,more reliable and effective sperm selection procedure.Methods 98 specimens from 68 sterility couples were collected.The specimen were detected by the old selection technology and the modified technology ( Direct swim-up and Discontinuous density-gradients ).Neubauer Blood Cell counter was used to detect the sperm concentration.Spain SCA-CASA was used to assess sperm motility and Papanicolaou Staining was used to detect sperm morphology.Results The forward sperm quantity and recovery improved after modification ( P＜ 0.05 ).The sperm concentration and normal forms of sperm did not change after modification ( P ＞ 0.05 ).The count of sperm with normal forms increased after selection ( P ＜ 0.01 ).Conclusions Modified sperm selection technology can improve the quantity of forward sperm and the recovery rate.It can increase the chance of conception in sterility couples. Key words: Sperm Selection;  Modification;  Male sterility