2018•Zhonghua xiaoerwaike zazhiRequires access
Effect of LncRNA CRNDE on the proliferation, apoptosis and migration of brain glioma cells
Wenyue Deng, Shiyuan Jing, Qing-Li Suo, Ruilong Gong, Yingying Lu
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Abstract
Objective
To explore the effect of LncRNA CRNDE on the proliferation, apoptosis and migration of brain glioma cells.
Methods
LncRNA CRNDE was interfered by SiRNA in U251 cells.PE Annexin V-FITC staining and CCK-8 assay were employed for observing the effect of interfering LncRNA CRNDE on cell proliferation and apoptosis.And the capacity of cell migration was detected by scratch test.
Results
After 12 h transfection, statistically significant difference existed between control and SiRNA (si783, si809) groups.However, there was no statistically significant difference between SiRNA (si783, si809) and non-transfected groups.After 24 h, 48 h transfection, statistically significant difference existed between SiRNA (si783, si809) group and control and non-transfected groups (P 0.05). Cell migration experiment indicated that cell count of transfected group (287.2±28.6) was lower than non-transfected group (518.2±29.9) and control group (471.6±29.4) (P 0.05).
Conclusions
CRNDE can effectively enhance cell proliferation and migration of glioma cells and decrease the rate of cell apoptosis.
Key words:
Glioma; Cell proliferation; Apoptosis; Cell migration assay; LncRNA CRNDE