2017•Unpublished venueRequires access
Changes and significance of CD5+ B lymphocyte in patients with systemic lupus erythematosus
Junwei Chen, Xiaona Jing, Meihua Hao, Zhaoyun Liang, Jinhua Yang, Meng Wu, Shaoliang Jie, Sheng‐Xiao Zhang, Yanfang Chu, Chunmiao Zhao, Jing Wang
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Abstract
Objective
To measure the number of lymphocytes, B lymphocytes, CD5+ B lymphocytes and level of IL-10 in peripheral blood of patients with systemic lupus erythematosus (SLE), and analyze their effects in the disease.
Methods
In this study, 84 cases of patients with SLE were randomly selected and evaluated according to the activity index (SLEDAI). These cases were divided into low activity group (SLEDAI<9) and high activity group (SLEDAI≥9). Ten healthy individuals were selected as the control group at the same time. The number of peripheral blood lymphocytes, B lymphocytes, CD5+ B lymphocytes, erythrocyte sedimentation rate (ESR),C3, C4 and interleukin (IL)-10 levels in serum were measured respectively and the correlation between the above indexes and SLEDAI and complement levels were analyzed. Pair-wise comparison of means of groups was conducted with one-way ANOVA. Comparison between the two groups was conducted by LSD-t test. Correlations between variables were carried out using Spearman's rank correlation test.
Results
The total number of lymphocytes in SLE group was lower than that in normal control group (F=7.216, P 0.05). In addition, the level of serum IL-10 in whether the low activity group(t=1.935, P=0.031) or the high activity group (t=3.048, P=0.012) was all higher than the normal control group. The level of serum IL-10 in patients with systemic lupus erythematosus was positively associated with SLEDAI score (r=0.425, P=0.024) and ESR (r=0.479, P=0.008), but was negatively correlated with complement 4 (r=-0.359, P=0.031).
Conclusion
The total number of lymphocytes in patients with SLE decreases significantly, while B lymphocytes increases significantly. The number of CD5+ B lymphocytes and the serum IL-10 level are also changed. It maybe related to the patient's inflammatory environment, and the number of CD5+B lymphocytes and the serum IL-10 level may be associated with disease activity.
Key words:
lupus erythematosus, systemic; B-lymphocyte subsets; Interleukin-10