The expression changes of miR-199a in hypoxic-induced pulmonary artery smooth muscle cells of rats

Objective To observe and analysis the changes of microRNA-199a(miR-199a) expression in rat pulmonary artery smooth muscle cells(PASMCs)induced by different hypoxia time,and lay the foundation to explore the effect and mechanism of regulation of miR-199a on PASMCs proliferation. Methods The primary cultured PASMCs respectively cultured under normoxic and hypoxic 6 h,12 h,24 h,48 h,using methyl thiazolyl tetrazolium colorimetric(MTT)detection of cell proliferation, real-time quantitative PCR detection of miR-199a expression in each group PASMCs. Results ①MTT test results showed that the PASMCs absorbance(A value) of the hypoxic group at each time point is significantly higher than the corresponding normoxic group(P< 0.05).②The expression of miR-199a in hypoxia group PASMCs was sustained with time decreased apart from hypoxic hypoxia 24 h group compared the expression of miR-199a 48 h group was no significant difference(P= 0.10),the expression of miR-199a among other groups PASMCs was significantly different(P< 0.05). The expression quantity of miR-199a of N48 h group,H6 h group,H12 h group,H24 h group,H48 h group is 5.55± 0.52,4.73±0.25,3.41±0.38,1.32±0.07,0.84±0.16,respectively. Conclusions The expression of miR-199a in PASMCs decreases after induction of hypoxia.We speculated that miR-199a may be involved in the regulation of hypoxia-induced PASMCs proliferation. Key words: MicroRNA-199a; Hypoxia; Pulmonary artery smooth muscle cells; Proliferation