The detection of procoagulant activity of tissue factor in patients with type 2 diabetic nephropathy and its clinical significance

Objective To detect the expression level and procoagulant activity of tissue factor (TF)in the blood and urine in patients with type 2 diabetic nephropathy (DN),and investigate its related clinical significance.Methods A total of 120 DN patients were collected and divided into 3 groups according to urinary albumin and creatinine ratio:normal albuminuria group (＜3.4 mg/mmol) with 64 cases,microalbuminuria group ( ≥3.4 mg/nunol,and ＜ 34.1 mg/mmol) with 35 cases and macroalbuminuria group (≥34.1 mg/mnol) with 21 cases.Twenty healthy persons were enrolled as control group.The expression of monocytes TF in peripheral blood was detected by flow cytometric analyzer.The expression ofTF in the blood and urine was detected by ELLSA.The prcoagulant activity of TF was detected by one-stage coagulation.Results The expressions of monocytes TF in normal albuminuria group,microalbuminuria group and macroalbuminuria group [ (4.32 ± 1.25 )％,(5.01 ± 1.73)％,( 1 1.83 ± 2.14)％ ] were significantly higher than that in control group [(0.84 ± 0.36)％ ](P ＜0.01).TF procoagulant activity in normal albuminuria group,microalbuminuria group and macroalbuminuria group [ ( 64.3 ± 21.2),( 67.1 ± 17.3 ),( 113.8 ± 44.4)mU/L] were obviously higher than that in control grouP [ (23.8 ± 16.4) mU/L](P＜ 0.01 ).The expression of monocytes TF in normal albuminuria group and microalbuminuria group had no statistical significance (P＞0.05),while the expression in macroalbuminuria group had significant difference compared with that in normal albuminuria group and microalbuminuria group (P ＜ 0.01 ).The expression of TF in the blood and urine of DN patients was obviously higher than that of control group (P ＜ 0.01 ).The expression of TF in the blood was positively correlated with that in urine (r =0.684,P ＜ 0.01 ).Conclusions The expressions of TF in the blood and urine in DN patients increase and its procoagulant activity enhances.The expression level of TF has a closed relationship with the increase of albuminuria. Key words: Diabetic nephropathies; Thromboplastin; Diabetes mellitus,type 2