Effects of sevoflurane anesthesia on learning and memory function and S100β protein in the 7-day-old rat

Objective To investigate the effects of sevoflurane anesthesia on learning and memory functions and S100βprotein in anaplasis in the 7- day- old rat. Methods 48 SD rats of both sexes aged 7- day- old weighing 12- 16 g were randomly divided into 3 groups(1 6 ratsin each group): group A and group Binhaled 3% sevoflurane in oxygen(1L/min) for 6h and 2h respectively;group C inhaled oxygen(1L/min) only. After inhalation, the effects of sevoflurane anesthesia on learning and memory function were assessed by Morris water- maze test and Y- maze test from 16d to 24d.The rats were killed in the day of 8d and 25d respectively,and the blood were collected for the expression of serum concentration S100βprotein by enzyme- linked immunosorbent assay. Results (1)Results of Morris water- maze test: Compared with group C,escape latency prolonged in group A and group Bin 17- 20d(P 0.05).(2)Result of Y- maze test:In 22d,the total reaction time of group A and B were longer than group C(P 0.05).(3)Results of the blood serum index: In 8d,serum concentration of S100β protein was significantly increased in group A and B compared with group C(P 0.05). Conclusion Sevoflurane anesthesia in the 7- day- old rat can temporarily decrease the ability of learning and memory functions in the length of inhalation time dependent manner, and the mechanism may be related to the increased expression of serum concentration of S100β protein transiently. Key words: Sevoflurane; Anesthesia; Learning; Memory; Protein,S100β