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Derivation,culture and in vitro differentiation of human embryonic stem cells

盖慧, 褚建新

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Abstract

胚胎干细胞具有体外无限增殖和分化成三胚层细胞的潜能,它已被视为治疗多种疾病的一种新兴策略.目前胚胎干细胞常规的建系和培养技术已很成熟,并有一套国际公认的鉴定标准.但常规方法存在异源病原体污染的可能,急需研究适于标准化、无动物源性污染及可大量培养胚胎干细胞的培养体系.在现阶段,通过不同的体外诱导途径可将胚胎干细胞诱导成为胚外和三胚层来源的各种细胞,但定向分化的问题仍亟需解决。

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What this paper is about

胚胎干细胞具有体外无限增殖和分化成三胚层细胞的潜能,它已被视为治疗多种疾病的一种新兴策略.目前胚胎干细胞常规的建系和培养技术已很成熟,并有一套国际公认的鉴定标准.但常规方法存在异源病原体污染的可能,急需研究适于标准化、无动物源性污染及可大量培养胚胎干细胞的培养体系.在现阶段,通过不同的体外诱导途径可将胚胎干细胞诱导成为胚外和三胚层来源的各种细胞,但定向分化的问题仍亟需解决。

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Available abstract

胚胎干细胞具有体外无限增殖和分化成三胚层细胞的潜能,它已被视为治疗多种疾病的一种新兴策略.目前胚胎干细胞常规的建系和培养技术已很成熟,并有一套国际公认的鉴定标准.但常规方法存在异源病原体污染的可能,急需研究适于标准化、无动物源性污染及可大量培养胚胎干细胞的培养体系.在现阶段,通过不同的体外诱导途径可将胚胎干细胞诱导成为胚外和三胚层来源的各种细胞,但定向分化的问题仍亟需解决。

Key concepts: Embryonic stem cell, Cell biology, In vitro, Stem cell, Biology, Cellular differentiation, Genetics, Gene

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