Correlation between txpression changes of vascular endothelial growth factor gene in human gliomas and Hedgehog/Gli signaling pathway

Objective To investigate the correlation between Hedgehog/Gli signaling pathway and tumor angiogenesis in glioma,and analyze the correlation between vascular endothelial growth factor (VEGF) expression and Hedgehog/Gli signaling pathway regulation of glioma.Methods In 54 cases of resected specimens from patients with glioma after surgery,immunohistochemistry was used to detect the expression of CD34 and Gli1 proteins to explore the relationship between the Hedgehog/Gli1 pathway and tumour angiogenesis.Extracellular inhibiting ligand Cyclopamine was used to repress the Hedgehog/Gli1 pathway in U87 and SHG4 cells in order to explore whether repression of this pathway could upregulate or downregulate the expression of VEGF.Results Gli1 expression was positively related with microvessel density (MVD) in glioma.Overactivation of the Hedgehog/Gli1 pathway effectively upregulated the VEGF expression,while repression of the Hedgehog/Gli1 pathway activity promoted downregulation of the VEGF expression.Conclusion Status of the Hedgehog/Gli1 pathway activated is correlated with angiogenesis in gliomas.The Hedgehog/Gli1 pathway can effectively regulate the expression of VEGF. Key words: Glioma; Vascular endothelial growth factor ; Signaling pathway