Dynamic changes of plasma LA and KIM-1 in rabbits with traumatic shock and the intervention of methylene blue

Objective To observe the changes of plasma lactic acid( LA ) and kidney injury molecule-1 ( KIM-1 ) in rabbits with traumatic shock and the intervention of methylene blue (MB),a guanylate cyclase inhibitor. Methods Eighteen rabbits were randomly divided into 3 groups(n=6):control group, traumatic shock resuscitation with normal saline group( NS group )and traumatic shock resuscitation with methylene blue group ( MB group). LA and KIM-1 of plasma in NS group, MB group and control group were measured at pre-shock, post-shock, post-resuscitation, 2, 4, 6 h and 8 h after resuscitation respectively. Results The levels of plasma LA and KIM-1 in rabbits after traumatic shock were very higher than that of pre-shock (P＜0.05). At 2,4,6 h and 8 h after resuscitation, the levels of plasma LA in NS group were (82.2±3.5), (93.6±2.6), ( 106.5±5.3 ) and ( 122.6±4.8 ) mmol/L, and the levels of plasma KIM -1 were (8.48 ±0.31 ), (9.29 ±0. 18 ), ( 9.88 ±0.17 ) and ( 10.74 ±0.45 ) ng/L. But after resuscitation. But after intravenous administration of MB, the levels of plasma LA were (74.7±3.4), (84.7±2.4), (94.8±2.6) and (106.8±5.2) mmol/L and the levels of KIM-1 were( 7.53±0.32 ), ( 8.60±0.34 ), ( 9.41 ±0.31 ) and ( 1 0.04±0.09 ) ng/L respectively, which were obviously lower than that of NS group (P＜0.05). In control group, there weren't significant changes about the levels of LA and KIM-1 among all courses.Conclusion MB can obviously ameliorate the function of kidney and protect the renal tissues in traumatic shock rats. Key words: Traumatic shock ;  Kidney injury molecule-1 ;  Methylene blue ;