Effect of antisenseoligodeoxynucleotide on cell proliferation and telomerase activity in K562 cell line

：Objective To observeanti-sense phosphorothioate oligonucletide (ASPSODN) targeted directly to hTERT mRNA toits inhibiting effect on aimed gene and the influence on the telomerase activity, cellularproliferation, cell apoptosis of K562 cells. Methods Human leukemia cell line K562 wastransfected with anti-sense oligonucleotide ASPSODN by liposome. The proliferationactivity of K562 cell line was determined by using methyl thiazolyl tetrazolium assay, andtelomerase activity was detected by TRAP-PCR-ELISA. Flow cytometry was adopted to examineapoptotic rate and cell cycle. RT-PCR was used to detect the expression of target genehTERT mRNA. Results 0.6 μmol/L ASPSODN (0.42 ±0.16) was remarkably decreasedthe expression of hTERT mRNA, Telomerase relative activation was decreased by 52 %.According to 0.6 (μmol/L ASPSODN caused significant the inhibition ofK562 cell growth. Apoptotic rate and cell cycle was examined by 0.6 μmol/LASPSODN with flow cytometry. The cell apoptosis rate of 0.6μmol/L ASPSODN were 10.31 %. It showed the cells treatedwith 0.6 uU PSASODN arrested in G_1/C_0. The ratio of cells in G_2/M and S period wasreduced. But there was no characteristic apoptosis peak. Conclusion ASPSODN targeted hTERTcan inhibit the expression of target gene hTERT mRNA, and decrease the telomerase activityof K562 cells. ASPSODN can inhibit strongly the proliferation of K562 cell and induce cellapoptosis by decreasing telomerase activity. Key words: Telomerase;  Oligoribonucleotides, antisense;  Cell cycle; K562 cell line