Effect of phosphodiesterase inhibitor Ro 20-1724 on social interaction ability of developing rats after repeating ketamine anesthesia

Objective To observe the effect of Ro 20-1724 on social interaction ability of developing rats after repeated ketamine anesthesia. Methods 32 rats with 21 days old were randomly divided into four groups, control group (C group), ketamine group (K group), ketamine + Ro 20-1724 group (K + R group), ketamine + ethanol group (K + E group). Ethanol was used as a solvent of Ro 20-1724. Ketamine 70 mg·kg-1 was intraperitoneal injected, 30 min later, to give or not give Ro 20-1724 0.5 mg·kg-1 or equivalent ethanol solvent for once each day for seven consecutive days.Then the rats were fed for three days.On the fourth day after the last administration, the social interaction ability were assessed in all rats. The expression of brain-derived neurotropic factor (BDNF) in hippocampal CA1 region was detected using conventional ELISA. Results Comparing with rats in C group, the time spent on the cage of lifeless body ((60±29)min vs (109±33)min, P 0.05). The expression of BDNF in hippocampal CA1 region was significantly lower in K group, comparing with C group ((8.6±2.7)ng/ml vs (11.8±2.4)ng/ml, P<0.01); and there was a significant increase in K+ R group, comparing with K group ((10.1±3.6)ng/ml vs (8.6±2.7)ng/ml, P<0.05). Conclusion Ro 20-1724 significant rescued social interaction impairment induced by ketamine anesthesia in developing rats. And BDNF in hippocampal CA1 region contribute to the reversal process. Key words: Ro 20-1724; Ketamine; Developing rats; Social interaction ability