Construction and expression of the eukaryotic expression system of fibroblast activation protein

Objective To construct a eukaryotic expression system of fibroblast activation protein (FAP) gene and detect its expression. Methods The polymerase chain reaction (PCR) primer was de-signed based on the full sequence of mFAP in GenBank (NM_007986). The mFAP gene was inserted into the eukaryotic expression vector pcDNA6/myc-His-B. The total RNA was extracted and re-transdueted into cDNA from the gastrocnemius on the 1st, 3rd, 5th and 7th day after the vector was inoculated into the tail vein of BALB/C mice. Results The results showed that the positive cloning of mFAP recombinant could express the FAP protein accurately and stably in the eukaryotes, and the biggest amount of FAP gene (0.841±0.040) and protein (85.380 ±4.425)% expression in BALB/C mice appeared on the 5th day, which was significant differ-ence from blank control group (P < 0. 01 ). Conclusion The recombinant eukaryotic expression system has been constructed successfully and accurately. Key words: Fibroblast activation protein;  Eukaryotic expression vector