2009•Zhonghua shouwaike zazhiRequires access

Application of melanocyte growh medium in the culture of Schwann cells from adult SD rats:an experimental study

Shaohua Zhang, Jie Lao, Yanfeng Li, Yudong Gu, Xin Zhao, Jifeng Li

Abstract

Objective To develop a simple and effective method to obtain highly purified Schwann cells. Methods After enzymatic digestion of Predegenerated sciatic nerves from 20 adult SD rats were with 1 week ion, the suspension were cultured in group A (DMEM/F12 medium) and group B (melanocyte growth medium) . cold jet as the passage method. S-100 stain for detection of Schwann cell purity. Results The cell yields and purity of group B was beyond 90%. Conclusion The combination of melanocyte growth medium and cold jet provides a convenient mean to obtain considerable Schwann cells with purity. Key words: Schwann cells; Cell culture techniques; Melanocyte growth medium

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Key concepts: Schwann cell, Melanocyte, Suspension culture, Cell culture, Medicine, Staining, Molecular biology, Cell biology