Application of High Performance Liquid Chromatography Method for Triadimenol Analysis in Water Sample

Abstract Triadimenol is the most important triazole fungicide used for prevention and treatment of plant fungal disease. A simple high performance liquid chromatography (HPLC) method for triadimenol analysis in water sample is reported in this study. The optimized HPLC system using C18 column was achieved using mobile phase composition containing acetonitrile-water (70:30, v/v), a flow rate of 1.0 mL/min, UV detection at 220 nm, and analysis time within 2.5 min. The calibration graph was linear in the range 5 to 50 mg/L with r2 of 0.9997. The limit of detection (LOD) and limit of quantification (LOQ) obtained were 1.23 and 4.12 mg/L, respectively. Combination of solid phase extraction (SPE) and the optimized HPLC method has been successfully applied for the determination of triadimenol in water sample with a recovery of 94.24 % (RSD = 5.38%, n = 4). The proposed HPLC method provides short analysis time, high reproducibility and high sensitivity.