2001•中华放射医学与防护杂志Requires access
The changes of CD34+ cells in C57 mouse bone marrow after irradiation and their roles in dysfunction of hematopoiesis
马增春, 高月, 刘永学, 谭洪玲, 张立, 陶来宝, Peng Chen
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Abstract
Objective To observe the changes of CD34+ cells in C57 mouse bone marrow after irradiation and investigate the role of apoptosis in radiation-induced dysfunction of hematopoiesis. MethodsFlow cytometric enumeration of CD34+ hematopietic stem and progenitor cells by double fluorescent labeling apoptosis detection by Annexin V-FITC kit,and cell cycle detection by PI labeling were carried out. Results ①Compared with the normal group,the percentage of CD34+ cells in bone marrow nucleated cells decreased at least for 14 days after irradiation,and the changes were related with irradiation doses.②At 6 h after irradiation,the largest amount of apoptopic cells could be detected.③Bone marrow cell cycle was perturbed after 5.5 Gy irradiation. Conclusion The percentage of CD34+ hematopietic stem and progenitor cells in C57 mouse bone marrow decreased after irradiation,and apoptosis might be responsible for the changes of the bone marrow cells.