2016•Zhonghua xiaohua zazhiRequires access
Expression of chemokine ligand 12/chemokine receptor type 7 in intestinal type gastric cancer and its relationship with lymph node and liver metastasis
Qi Xin, Qin Zhang, Na Zhang, Likun Wen, Guiqiu Liu, Chuanshan Zhang
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Abstract
Objective
To investigate the expression of chemokine ligand 12(CXCL12)/chemokine receptor type 7(CXCR7) in intestinal type gastric cancer and its relationship with clinical pathological characteristics and lymph node, liver metastasis.
Methods
Sixty cases of intestinal type gastric cancer and its carcinoma adjacent tissues, 30 cases of intestinal type gastric cancer with lymph node metastasis and 20 cases with liver metastasis were selected as study subjects. The expression of CXCL12 and CXCR7 in gastric cancer tissues, its adjacent tissues and metastatic lymph nodes and liver tissues was detected by immunohistochemistry.The relationship between CXCL12, CXCR7 expression in intestinal type gastric cancer and clinical pathological characteristics, lymph node and liver metastasis were analyzed. The relationship between CXCL12+ CXCR7+ , CXCL12+ CXCR7- or CXCL12-CXCR7+ , CXCL12-CXCR7-gastric cancer tissue and tumor size, depth of invasion, clinical stage as well as lymph node, and liver metastasis were further analyzed. McNemar test, Chi square test and Spearman rank correlation analysis were performed for statistical analysis.
Results
CXCR7 expressed in intestinal type gastric carcinoma and was positively correlated with the expression of CXCL12 (Kappa=0.321, P=0.010). The expression of CXCR7 in intestinal type gastric carcinoma was correlated with lymph node and liver metastasis, tumor size, depth of invasion and clinical stage (χ2=5.879, 7.547, 5.701, 7.699 and 4.434, all P<0.05). Lymph node and liver metastasis were more common in CXCL12+ CXCR7+ gastric cancer tissues than those of CXCL12+ CXCR7-, CXCL12-CXCR7+ , and CXCL12-CXCR7- gastric cancer (r=0.586, P<0.01; r=0.275, P=0.033). The expression of CXCL12+ CXCR7+ were stronger in tumor maxium diameter no less than 5 cm, depth of invasion T3+ T4 and clinical stage Ⅲ+ Ⅳ intestinal type gastric cancer.
Conclusion
CXCL12/CXCR7 could be an biological axis in proliferation, invasion, as well as lymph node and liver metastasis of intestinal type gastric cancer.
Key words:
Intestinal type gastric cancer; Chemokine ligand 12; Chemokine receptor 7; Immunohistochemistry