2019•国际医药卫生导报Requires access
Effect of PI3K-Akt agonist 740 Y-P on INS-1 cells and its mechanism
Siting Zhao, Yangyang Wang, Yutian Chen, Liqiang Chen
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Abstract
Objective
To investigate the effect of PI3K-Akt agonist 740 Y-P on the expression of Akt and Caspase3 protein in rat pancreatic β-tumor cells (INS-1) and its significance.
Methods
INS-1 cells were stimulated with PI3K-Akt agonist 740 Y-P, and the expression levels of p-Akt and Caspase3 protein were detected by Western blotting.
Results
The results showed that 740 Y-P significantly increased the protein expression of p-Akt, but decreased the expression of Caspase3 (both P<0.05).
Conclusion
PI3K-Akt agonist 740 Y-P could induce the proliferation of INS-1 cells, which maybe one of the mechanism about pancreatic beta cell apoptosis in type 2 diabetes.
Key words:
Type 2 diabetes; 740 Y-P; Cell apoptosis