Express of Notch1 protein in adult adenocarcinoma of lung and its significance

Objective To investigate the expression of Notch1 and its role in the pathogenesis in adult adenocarcinoma of lung.Methods The high expression of Notch1 was detected in lung adenocarcinoma in our previous study.In this investigation,operation specimens of adenocarcinoma of lung that were proved by pathological sections were collected.Notch1 mRNA and protein in lung adenocarcinoma were measured with real-time fluorescence quantitative PCR and Western blot respectively to investigate the role of Notchl in adenocarcinoma of lung.Results It was shown by real-time fluorescence quantitative PCR that the expression of Notch1 mRNA in lung adenocarcinoma group (1.99+1.40) was higher than that in normal lung group (1.50+1.03) ( P ＜0.05).The expression of Notch1 protein in lung adenocarcinoma group (0.64+0.25) was significantly higher than that in normal lung group ( 0.14 + 0.07 ) ( P ＜ 0.01 ) by Western blot.Conclusions Notch1 signal may play a stimulative role in the pathogenesis of lung adenocarcinoma. Key words: Lung adenocarcinoma; Notch1; Real-time fluorescence quantitative PCR