2014•Zhonghua neifenmi daixie zazhiRequires access
Role of RIP140 in the H2O_2-induced injury of MIN6 cells
Junli Xue, Deng Haohua, Zeng Jiaoe, Xiaoqi Chen, Zhe Dai, Zou Runmei, Sun Jiazhong, Yancheng Xu
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Abstract
MIN6 cells cultured with 400 μmol/L H2O2 were divided into normal control group,control group with 400 μmol/L H2O2,scrambled siRNA group,and RIP140 siRNA group.400 μmol/L H2O2 could significantly increase apoptosis rate of MIN6 cells(27.89％ ± 1.91％ vs 3.74％ ±0.55％,P＜0.01) and the content of maleic dialdehyde [M DA,(35.17 ± 6.26 vs 20.07 ± 1.86) nmol/mg,P＜0.05],suppress cell viability (24 and 48 h,both P＜0.01),and lower the level of total superoxide dismutase [SOD,(4.79 ± 0.85 vs 3.09 ± 0.60) U/mg,P＜ 0.05].When receptor interaction protein 140 (RIP140) expression was suppressed by RNA interference,the apoptosis rate of MIN6 cells (11.45 ％ ± 2.27％ vs 30.39％ ± 5.32％,P＜0.01) and the content of MDA [(22.32 ± 1.94 vs 33.42 ± 2.51) nmol/mg,P＜0.01] were reduced,and levels of total SOD was increased [(5.32 ± 0.40 vs 3.09±0.15) nmol/mg,P＜0.01)].
Key words:
Receptor interaction protein 140; Hydrogen peroxide; MIN6 cells; Proliferation; Apoptosis