Ozone oxidative intervention protects against hepatic ischemia reperfusion injury in rats

Objective To explore the protective effect of ozone oxidative preconditioning on hepatic ischemia reperfusion injury in rats. Methods Eighteen 8-week-old SPF Sprague-Dawley male rats weighting 250~300 g were randomly divided into three groups (n=6 each): sham operation group (group S), ischemia/reperfusion group (group I/R) and ozone oxidative preconditioning group (group O3+ I/R). In Group O3+ I/R, rats received five-day preconditioning treatments by intraperitoneal injection of ozone and oxygen mixed gases (ozone concentration 50 mg/L, 1 mg·kg-1·d-1), and then experienced the procedure of hepatic ischemia/reperfusion injury. Model of hepatic ischemia/reperfusion injury was established by clamping the branches of hepatic artery and portal vein in the median and left lateral hepatic lobes for 45 min, followed by 3-h reperfusion. After reperfusion, blood samples were taken from the aorta abdominalis for detecting serum aminotransferases (ALT & AST). These rats were executed and the hepatic tissue samples were collec-ted for measuring hepatic malondialdehyde (MDA) and superoxide dismutase (SOD) level. Results Compared with group S, concentrations of serum ALT, AST and hepatic MDA were increased in group I/R and O3+ I/R; concentrations of hepatic SOD were decreased (P<0.05) in group I/R, but concentration of hepatic SOD was increased in group O3+ I/R. Compared with group IR, concentrations of serum ALT, AST and hepatic MDA were decreased, while concentration of hepatic SOD was increased in group O3+ I/R (P<0.05). Conclusion Ozone oxidative preconditioning could inhibit the lipid peroxidation to protect the rats against hepatic ischemia/reperfusion injury. Key words: Ozone oxidative preconditioning; Ischemia/reperfusion injury; Liver