Expression of cytoglobin in brain tissues of neonatal rats with hypoxic-ischemic brain damage

Objective To study the expression of cytoglobin (CYGB) in the brain tissues of 7-day-old rats with hypoxic-ischemic brain damage (HIBD). Methods Seventy 7-day-old SD rats were divided into HIBD group(n=50),sham-operated group (n=10) and control group (n=10). HIBD group rats were HIBD models and subdivided into 0 h,4 h,12 h,24 h and 48 h groups after hypoxic-ischemic injury. Brain tissues of rats in sham-operated and control group were collected after operation immediately and brain tissues in HIBD group were collected at different times. Western blot and immunohistochemistry were used to detect the expression of CYGB. Results Immunohistochemistry test showed that CYGB was located in the cortex, thalamencephalon and hippocamp and stronger CYGB expression was found in the HIBD group in comparison with the other two groups. Western blot showed that the expression of CYGB at 0 h,4h,12h, 24 h and 48 h after hypoxic-ischemic injury (261. 5±5. 0,263. 0±5.4,464. 3±6.8,522. 8±11. 2,512. 9±7. 9) were significantly stronger than that in the sham-operated group (117. 6±7.0) and the control group (116. 6±9. 0)(P 0. 05). Conclusions CYGB may have important role in protecting the brain from hypoxic-ischemic injury. Key words: Globins;  Hypoxia-ischemia, brain;  Rats, Sprague-Dawley