Verification of a method for determination of iodine by As3+-Ce4+ catalytic spectrophotometry.

Objective To verify the feasibility and application value of arsenic-cerium catalytic spectrophotometry for determination of iodine in serum. Methods The blood samples of adults were collected and the iodine standard curve of 0 - 300 μg/L was prepared. Referring to The Method for Determination of Chemical Substances in Biomaterials, the present method was tested in terms of linear relationship, minimum detection limit, precision and accuracy. Results The correlation coefficient of the method was - 0.999 7 - - 0.999 4 (n = 6) and the minimum detection limit was 5.13 μg/L in the range of 0 - 300 μg/L standard curve. Precision verification displayed that the variable coefficient of three different iodine concentration serum samples were 0.89%, 1.88%, 0.67%. And the accuracy verification displayed that the recovery of iodine standard was 93.90% - 107.04%, and the total average recovery was 99.48%. Conclusion The method has been proved to be of good linearity, high precision and high accuracy in the determination of serum iodine, which meets the requirements of biological sample analysis. Key words: Serum; Iodine; Spectrophotometry; Method validation