Effect of endoplasmic reticulum stress against kidney injury in rat with sepsis

Objective To investigate effect of endoplasmic reticulum stress against kidney injury in rat with sepsis. Methods Thirty- two male SD rats were randomly divided into sham group, sepsis 6 h group, sepsis 12 h group and sepsis 24 h group, with 8 rats in each group.Sepsis was induced by cecal ligation and puncture, Eight rats were sacrificed at 6,6, 12 and 24 hours after operation respectively in each group. Levels of blood urea nitrogen(BUN) and Serum creatinine(Scr) were detected.Apoptotic index of kidney issue were measured by terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL), and expression levels of glucose regulated protein 78 (GRP78), CCAAT/enhancer- binding protein homologous protein(CHOP) and caspase- 12 protein and m RNA was assayed respectively by Western blotting and reverse transcriptase- polymerase chain reaction(RT- PCR). Results Compared to sham group, levels of BUN[(7. 54±1. 62) mmol/L vs.(5. 26±0. 34) mmol/L], Scr[(57. 14±9. 53)μmol/L vs.(42. 35±4. 71)μmol/L], AI of kidney issue[(25. 7±2. 8)% vs.(2. 9±0. 6)%]and expression levels of GRP78, CHOP and caspase- 12 protein[(0. 22±0. 03) vs.(0. 14±0. 02),(0. 16±0. 04) vs.(0. 07±0. 01),(0. 24± 0. 03)vs.(0. 11±0. 02)]and m RNA[(0. 31±0. 08)vs.(0. 21±0. 06),(0. 33±0. 04)vs.(0. 26± 0. 03),(0. 39±0. 06) vs.(0. 22±0. 02)] were increased(P< 0. 05). Levels of BUN, Scr, AI of kidney issue and expression levels of GRP78, CHOP and caspase- 12 protein]and mRNA were increased in both sepsis 12 h group and sepsis 24 h group compared to sepsis 6 h group(P< 0. 05), and these index were higher in sepsis 24 h group than in sepsis 12 h group. Conclusion There exist association between septic kidney injury and endoplasmic reticulum stress- induced apoptosis in kidney issue. Key words: Sepsis; Kidney injury; Endoplasmic reticulum stress; Apoptosis