The effects of Biocoen on HIF-1α expressions in myocardium of rats after cardiopulmonary resuscitation

Objective To investigate the effects of Biocoen on HIF-la expression in myocardium and protection from cardiac injury in SD rats after cardiopulmonary resuscitation(CPR).Method Seventy-two male SD rats were randomly(random number)divided into three groups:sham control group,routine treatment group (0.5,2,4 and 6 h subgroups)and Biocoen intervention group(0.5,2,4 and 6 h subgroups).Cardiac arrest was induced by sueeinylcholine to render rats asphyxiated along with ice-cold 0.5 moL/L KCI injection,and resuscitation was initiated in five minutes.In Biocoen intervention subgroups diluted Bioeoen was intraperitoneally injected when their heartbeats resumed.The Western blotting method was used to assay HIF-1α_ protein in eardiomyoeytes in all groups.The eolorimetry was used to determine the quantity of malondialdehyde(MDA)and activity of superoxide dismutase(SOD),and Na~+-K~+-ATPase in cardiomyocytes.One-way ANOVA was used for comparison among multiple groups,and SNK-q test was used for comparison between two groups.Results Compared with the sham group,HW-1_α protein in routine CPR groups was significantly increased(P＜0.05)and peaked at 2 hours after ROSC(0.15±0.02 in sham group)in routine group(0.57±0.06),and the quantity of MDA significantly increased(P＜0.05)along with notably declined activity of SOD and Na~+-K~+-ATPase(P＜0.05).Compared with the routine CPR groups,HIF-1_α protein in all Biocoen groups were decreased(P＜0.05)with the most significant differences between these two-hour groups[(0.29±O.03)in Biocoen intervention group vs.(0.57±0.06)in routine CPR group]along with notably increased activity of SOD and Na+-K+-ATPase (P＜0.05).Conclusions HIF-1α protein enhanced in myocardium cells of rats after CPR.Biocoen can enhance Na~+-K~+-ATPase activity and reduce the level of HIF-1_α protein in myocardial cells.Meanwhile,Bioccen can lessen the injury of myocardial cells caused by ischemia-repetfusion injury,and plays a pivotal role in protection of the heart after CPR. Key words: Cardiopulmonary resuscitation; Biocoen; Myocardial cell; lschemia-reperfusion; Hypoxia-inducible factor 1α