Therapeutic effects of transplantation of human amnion - derived mesenchymal stem cells in rats with spinal cord injury

Objective To study the effects of transplantation of human aminon -derived mesenchymal stem cells on the neurological function restoration of rats with spinal cord injury.Methods Transected spinal cord injury(SCI) model in rats was established.hAD - MSCs (about 2 × 105 ) or equal volume of PBS absorbed by a gelfoam were transplanted into the interval of injury ends immediately after SCI.The movement function of hindlimbs was evaluated by Basso-Beattie-Bresnahan(BBB) scorse every week after SCI.The survival and differentiation of hAD-MSCs in the injured spinal cord were evaluated by immunofluorescence staining.The expression of NF-200 in the distal end of injured site was detected with immunohistochemical staining.Results The neurological function in hAD- MSCs transplantation group was improved significantly and the BBB score was increased gradually ( P ＜0.05).The grafting hAD -MSCs were MAB1281postively at the 2nd week after transplantation,but were not MAP-2 and GFAP postively.The expression of NF -200 in the distal end of injured site of spinal cord of hAD -MSCs transplantation group was remarkably higher than that of PBS control group after transplantation.Condusions Transplantation of hAD - MSCs could be benefit to improve neurological function restoration of rats with SCI.Its mechanism might relate to the promotion of NF- 200 expression in the distal end of injured spinal cord after hAD-MSCs transplantation. Key words: Rats; Spinal cord injury; Transplantation; Human amnion - derived mesenchymal stem cell