The inhibitory effects of an antisense survivin vector on proliferation and invasion of osteosarcoma cells

Objective To observe the inhibitory effects of an antisense Survivin vector on proliferation and invasion of osteosarcoma cells line MG-63, and explore the mechanism. Methods MTT assay was used to measure the proliferation inhibition after transfection of MG-63 cells with Survivin antisense RNA. Boyden chamber in vitro invasion assay was applied to observe the changes of invasion capability.The expression of Angd-2 before and after transfection was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results The proliferation of cells transfected with antisense Survivin was significantly inhibited ( the inhibition rate was 23.4% ) as compared with other groups. The invasion ability was remarkably decreased after antisense transfection ( 17. 9% ) as compared with the control groups. Moreover,the mRNA expression of Ang-2 in antisense Survivin transfected cells was much lower than control groups.Conclusion The antisense Survivin vector could markedly inhibit the proliferation and invasion of osteosarcoma cells line MG-63, and partially reversed their malignant phenotype, which was possibly related with down-regulation of the Ang-2 expression. Key words: Osteosarcoma;  Survivin;  Ang-2