A new animal subarachnoid hemorrhage model

Objective To set up a new experimental model of subarachnoid hemorrhage.Methods A rabbit model was established by endovascular puncture in the internal carotid artery (ICA) through the femoral artery.60 rabbits were randomly divided into 10 groups (control,sham-operation,0 h,3 h,6h,12 h,1 d,3 d,7 d,and 14 d after SAH).After cerebral angiography at different time points,all rabbits underwent perfusion-fixation.The basilar artery cuts were stained with HE for morphologic examinations.The artery diameter and vascular wall thickness were determined by image analysis systems analysis.Results The biphasic process of CVS included acute phase spasms (12 h after SAH) and delayed phase spasms (7 d after SAH) in the rabbit model made by the endovascular puncture method.Conclusion The model made by the endovascular puncture method can simulate the pathologic process of CVS after SAH. Key words: Subarachnoid hemorrhage ;  Cerebral vasospasm ;  Animal model;  Rabbits