2009•Zhonghua chuangshang guke zazhiRequires access
Culture and differentiation of neural stem cells from the injured spinal cord in adult rats
Zheng-xu Ye, Jinghui Huang, Min Yan, Wei Liang, Man Gil Yang, Zhuojing Luo
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Abstract
Objective To investigate culture and differentiation of neural stem cells from the injured spinal cord in adult rats.Methods The spinal cord of male SD rats was injured by Allen's weight dropping on T8.Procedures of floating neural stem cell culture were carried out.Results Neurospheres of neural stem cells emerged after plating for 1 week.They had potent serf-renewing ability and could be passaged repeatedly,Immunohistochemistry data showed that these neurospheres strongly expressed nestin.A characteristic intermediate filament was seen in neural stem cells.The stem cells were further induced to differentiate into cells expressing β-tubulin,glial fibrillary acidic protein (GFAP) and myelin associated glycopretein (MAG),suggesting their differentiating potencies into neurons,astrocytes and oligodendrocytes.Conclusion In adult rats after spinal cord injury the neural stem cells can be isolated and cultured in vitro and induced to differentiate into neurons,astrocytes and oligodendrocytes,so the neural stem cells may participate in repair of spinal cord injury.
Key words:
Spinal cord; Spinal cord injuries; Stem cells; Cell differentiation; Rat