Association of interleukin-17 on the expression in the renal of rats with diabete and a smooth muscle actin

Objective To investigate the association between the expression of interleukin-17 (IL-17) in renal tubuleinterstitium and diabetic nephropathy.Methods 55 rats were randomly divided into three groups:control group,diabetic rat group.Diabetic rat group was injected 50 mg/kg streptozoticin (STZ).In the 2th,4th week,serum and urine IL-17 were measured with enzyme-linked immunosorbent assay (ELISA).The expression of IL-17,α-smooth muscle actin (α-SMA) and nuclear factor-κB p65 (NF-κB p65) in the kidney were assessed by immunohistochemisty staining and Western blotting.Results Serum and urine IL-17 in diabetic rats were [(11.51 ±5.62),(79.21 ± 14.53) ng/L],which were significantly upreguated as compared with those in control group rats (P ＜ 0.01),which were [(8.87 ± 3.35),(6.75 ± 1.13) ng/L].The expression of IL-17 was only observed in renal tubuleinterstitium of diabetic rats,the expression of IL-17 in renal tubuleinterstitium of diabetic rats was positively correlated with urine IL-17 (r =0.715,P ＜ 0.05),and the expression of α-SMA (r =0.708,P ＜ 0.05)、NF-κB p65 (r =0.638,P ＜ 0.01) in renal.Conclusion The effect of IL-17 on pathogenesis and progression of diabetic nephropathy in diabetic rat might be up-regulating the expression of α-SMA. Key words: Interleukin-17 ;  Diabetes, renal tubular-interstitial ;  α-smooth muscle actin ; Nuclear factor-kappa B