2012•Zhonghua jianyan yixue zazhiRequires access
Analysis of the expression in platelets membrane glycoprotein of the MYH9-RD family by flowcytometry
Shufang Zhang, Shun-lan Wang, Ying-ai Zhang, Jing-chuan Xiao
Open publisher page 1 citations
Abstract
Objective
To explore the pathogenic mechanism by detecting the expression of membrane glycoprotein in the platelets of nonmuscle myosin heavy chain 9 related disease (MYH9-RD) patients.
Methods
Periperal bloods were obtained from 11 MYH9-RD patients and 7 normal family members.Flow cytometry was used for detecting the expression of the platelet membrane glycoprotein including GPⅡb/Ⅲa(CD41/61) ,GPⅠa(CD49b) , GPⅠb/Ⅸ/Ⅴ（CD42a）GPⅠb(CD42b) and GPⅣ(CD36).
Results
The expression fluorescence intensity of platelet membrane glycoprotein GPⅡb/Ⅲa(CD41/61), GPIa(CD49b), GPⅠb/Ⅸ/Ⅴ（CD42a）GPⅠb(CD42b) and GPⅣ(CD36) are 653.7±192.7,420.0±151.3,667.7±371.3 and 236.4±64.2 respectively ,which are significantly higher than those in normal controls （406.7±126.1，181.2±29.3，271.4±91.6 and 136.1±23.5；P 0.05）.
Conclusion
In our study，the diverse clinical manifestations in patients with MYH9-RD is probably associated with the expression level of platelet membrane glycoprotein（Chin J Lab Med，2012,35：1159-1162）
Key words:
Thrombocytopenia; Platelet membrane glycoproteins; Myosin heavy chains; Molecular motor proteins; Flow cytometry