2008•Zhonghua gan-dan waike zazhiRequires access
Methylation of tumor suppressing genes in hepatocellular carcinoma
Xin Sun, Lixin Zhu, Bo Qian
Open publisher page 0 citations
Abstract
Objective To study the methylation state of RASSF1A,DAPK,FHIT,RIZ1,and p73 genes in heDatocellular carcinoma.Methods The technique of methylation-specific PCR was adopted to investigate the promoter methylation of these genes in 40 cases of HCC and 4 normal liver tissues.The relationship between the frequency of methylation of the genes and clinical data was analyzed.Results The phenomenon of genes methylation was absent in patients with normal liver.In the samples of HCC,the frequency of methylation in RASSF1A,DAPK,FHIT,RIZ1,and p73 were 90%,77.5%,70%,30% and 5%,respectively.In the non-tumor liver tissues,the frequency of methylation in RASSF1A,DAPK,FHIT,RIZ1,and p73 were detected in 77.5%,77.5%,62.5%,5% and 0%,respectively.Conclusions Methylation of multiple genes is a common event in HCC.
Key words:
Carcinoma hepatocellular; DNA Methylation; Tumor suppressing gene