Effect of microRNA-451 on proliferation and apoptosis of colon cancer cells via targeting regulation of c-Myc

Objective To explore effect of microRNA (miRNA, miR)-451 on proliferation and apoptosis of colon cancer cells via targeting regulation of c-Myc. Methods MiR-451 mimics and miR-451 NC were transfected into SW620 cell by liposome Lipofectamine™2000. The expression of miR-451 was detected by Real-time polymerase chain reaction (RT-PCR). Cell viability was measured by cell counting kit-8 (CCK-8) assay. Cell apoptosis and cell cycle was detected by flow cytometry. The expression of c-Myc protein and mRNA was measured by Western blotting and RT-PCR. Luciferase reporter analysis was performed. Results The expression of miR-451 in miR-451 mimics group (1.45±0.15) was higher than that in miR-451 NC group (0.36±0.03) (P<0.05). Cell viability in miR-451 mimics group (0.36±0.03) was lower than that in miR-451 NC group (0.63±0.06) (P<0.05). Cell early apoptotic rate (18.64±1.87)% and late apoptotic rate (17.83±1.78)% in miR-451 mimics group was higher than that in miR-451 NC group [ (2.46±0.24)%, (3.05±0.30)%, P<0.05], the G1 phage in miR-451 mimics group (64.88±6.48)% was higher than that in miR-451 NC group (50.49±5.04)% (P<0.05). The expression of c-Myc protein and mRNA was lower than that in miR-451 NC group (P<0.05), miR-451 mimics targeted regulation the expression of c-Myc. Conclusion miR-451 mimics could inhibit SW620 cell proliferation and induce cell apoptosis by targeting down-regulation expression of c-Myc. Key words: MicroRNA-451; Colon cancer cell SW620; Proliferation; Apoptosis