Inhibitive effect of human p66Shc recombinant adenovirus on proliferation of Hela cells

Objective To investigate the inhibitive effect and mechanism of human p66Shc recombinant adenovirus on the proliferation of human cervical cancer Hela cells.Methods The cells were divided into three groups:blank control group (Hela cells),negative control group (Hela cells infected with Adeno X-LacZ) and experiment group(Hela cells infected with Adeno X-p66Shc).The proliferation ability was investigated by cell growth curve,MTT detection.Reactive oxygen species (ROS) were detected by Fluorescent Probe-DHE(dihydroethidium).The cell cycles were analyzed by flow cytometry.Western blot were used to observe associated protein expression.Results The human p66Shc recombinant adenovirus could efficiently suppress the proliferation of Hela cells.The inhibition effects seem to be positive dose and time of action dependent.Compared with blank and negative control group,the ratio of the cell phase G2/M of Hela cells infected with Adeno X-p66Shc for 48h showed a significant increase.The cellular ROS,the expression of p53 and Cyclin B 1 proteins were upregulated in Hela cells.Conclusion Adeno X-p66Shc can effectively inhibit proliferation of Hela cells and induce cell cycle arrest at G2/M phase. Key words: p66Shc; Cervical cancer cells; Hela cells; Proliferation