Clinical analysis on the mobilization and collection of peripheral hematopoietic stem cells in 198 healthy donors

Objective To investigate the methods and effects on the mobilization and collection of peripheral hematopoietic stem cells. Methods Peripheral hematopoietic stem cells of 198 cases of healthy donors were selected and mobilized by subcutaneous recombinant human granulocyte colony stimulating factor (rhG-CSF) by (5-10) μg·kg-1 ·d-1 and collected on the 5th day. The effects of gender, height, age and WBC count of donors on mobilization and collection were analyzed. Results All the peripheral hematopoietic stem cells of donors were successfully mobilized. The average counts of mononuclear cells (MNC) and CD34+ cells were (4.19±1.96)×108/kg and (2.98±1.40)×106/kg, respectively, which had no correlation with gender, height and age. The counts of MNC and CD34+ cells collected were positively correlated with the WBC count of peripheral blood (r =0.9201, P =0.0035; r =0.8420, P =0.0149). The donors with WBC ≥20.0×109/L had moresignificant effect than those WBC＜20.0×109/L (F =4.688, P =0.0013; F =4.622, P =0.0006). Conclusion The WBC count of peripheral blood from healthy donors is a simple and feasible indicator to predict the quantity of CD34+ cells. Key words: Hematopoietic stem cell transplantation;  Hematopoietic stem cell mobilization;  Collection