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2013•Zhonghua weichan yixue zazhiRequires access

Effects of dexamethasone and oxidative stress on surfactant protein A, B, C and D expressions of H441 cells

Baohuan Cai, Wenbin Li, Wei Liu, Xijuan Wang, Lingxia Zhao, Luxia Mo, Jing Wang

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Abstract

Objective To explore the effects of dexamethasone on the expressions of surfactant protein(SP) A,B,C and D in H441 cells injured by oxidative stress.Methods H441 cells were challenged with hydrogen peroxide as oxidative stress damage cell model.Then the cells were divided into control group,groups on different dexamethasone levels (1,10 and 100 umol/L),H2O2 (100 μmol/L) injury group and dexamethasone (100 μmol/L)+ H2 O2 (100 μmol/1) group.All cells were cultured for 24 hours,then the expressions of SP mRNA were analyzed by quantitative real timepolymerase chain reaction,and proteins were detected by Western blot.One-way variant analysis and Dunneu's T3 test were applied as statistical methods.Results (1) The mRNA level of SP-A in H2 O2 injury group was higher than that in the control group (4.0016±0.3618 vs 0.9914±0.4170),while mRNA of SP C and SP-D were lower than those in the control group (SP C:0.2840±0.0586 vs 0.9719±0.1022;SP-D:0.6199±0.0074 vs0.9518±0.0614) (all P＜0.05).Compared with H2O2injury group,the mRNA level of SP-A in dexamethasone + H2O2 group was down-regulated (2.5191±0.3463),but SP-D mRNA was up-regulated (0.9076±0.0167)(all P＜0.05).(2) The SP-A protein level in H2O2 injury group was higher than that in the control group (2.1795±0.0645 vs 0.9728±0.009),while SP-B、SP-C and SP-D protein levels were lower than those in the control group (SP-B:0.2593±0.0086 vs 0.7253±0.0134;SP-C:0.5592±0.0111 vs 0.9472±0.0052;SP-D:1.1550±0.0237 vs 1.2991±0.0298).Compared with H2O2 injury group,the protein level of SP-A in dexamethasone+ H2 O2 group was down-regulated(1.4974 ± 0.0185),while SP-B,SP-C and SP-D levels were up-regulated (0.7389±0.0182,0.9244±0.0282 and 1.3339±0.0136).The differences were statistically significant respectively (all P＜0.05).Conclusions Dexamethasone may protect H441 cells from being damaged by H2O2 through regulating SP mRNA and protein expression. Key words: Oxidative stress;  Cell line, tumor;  Pulmonary surfactant-associated proteins; Dexamethasone

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Objective To explore the effects of dexamethasone on the expressions of surfactant protein(SP) A,B,C and D in H441 cells injured by oxidative stress.Methods H441 cells were challenged with hydrogen peroxide as oxidative stress damage cell model.Then the cells were divided into control group,groups on different dexamethasone levels (1,10 and 100 umol/L),H2O2 (100 μmol/L) injury group and dexamethasone (100 μmol/L)+ H2 O2 (100 μmol/1) group.All cells were cultured for 24 hours,then the expressions of SP mRNA were analyzed by quantitative real timepolymerase chain reaction,and proteins were detected by Western blot.One-way variant analysis and Dunneu's T3 test were applied as statistical methods.Results (1) The mRNA level of SP-A in H2 O2 injury group was higher than that in the control group (4.0016±0.3618 vs 0.9914±0.4170),while mRNA of SP C and SP-D were lower than those in the control group (SP C:0.2840±0.0586 vs 0.9719±0.1022;SP-D:0.6199±0.0074 vs0.9518±0.0614) (all P＜0.05).Compared with H2O2injury group,the mRNA level of SP-A in dexamethasone + H2O2 group was down-regulated (2.5191±0.3463),but SP-D mRNA was up-regulated (0.9076±0.0167)(all P＜0.05).(2) The SP-A protein level in H2O2 injury group was higher than that in the control group (2.1795±0.0645 vs 0.9728±0.009),while SP-B、SP-C and SP-D protein levels were lower than those in the control group (SP-B:0.2593±0.0086 vs 0.7253±0.0134;SP-C:0.5592±0.0111 vs 0.9472±0.0052;SP-D:1.1550±0.0237 vs 1.2991±0.0298).Compared with H2O2 injury group,the protein level of SP-A in dexamethasone+ H2 O2 group was down-regulated(1.4974 ± 0.0185),while SP-B,SP-C and SP-D levels were up-regulated (0.7389±0.0182,0.9244±0.0282 and 1.3339±0.0136).The differences were statistically significant respectively (all P＜0.05).Conclusions Dexamethasone may protect H441 cells from being damaged by H2O2 through regulating SP mRNA and protein expression. Key words: Oxidative stress;  Cell line, tumor;  Pulmonary surfactant-associated proteins; Dexamethasone

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Available abstract

Objective To explore the effects of dexamethasone on the expressions of surfactant protein(SP) A,B,C and D in H441 cells injured by oxidative stress.Methods H441 cells were challenged with hydrogen peroxide as oxidative stress damage cell model.Then the cells were divided into control group,groups on different dexamethasone levels (1,10 and 100 umol/L),H2O2 (100 μmol/L) injury group and dexamethasone (100 μmol/L)+ H2 O2 (100 μmol/1) group.All cells were cultured for 24 hours,then the expressions of SP mRNA were analyzed by quantitative real timepolymerase chain reaction,and proteins were detected by Western blot.One-way variant analysis and Dunneu's T3 test were applied as statistical methods.Results (1) The mRNA level of SP-A in H2 O2 injury group was higher than that in the control group (4.0016±0.3618 vs 0.9914±0.4170),while mRNA of SP C and SP-D were lower than those in the control group (SP C:0.2840±0.0586 vs 0.9719±0.1022;SP-D:0.6199±0.0074 vs0.9518±0.0614) (all P＜0.05).Compared with H2O2injury group,the mRNA level of SP-A in dexamethasone + H2O2 group was down-regulated (2.5191±0.3463),but SP-D mRNA was up-regulated (0.9076±0.0167)(all P＜0.05).(2) The SP-A protein level in H2O2 injury group was higher than that in the control group (2.1795±0.0645 vs 0.9728±0.009),while SP-B、SP-C and SP-D protein levels were lower than those in the control group (SP-B:0.2593±0.0086 vs 0.7253±0.0134;SP-C:0.5592±0.0111 vs 0.9472±0.0052;SP-D:1.1550±0.0237 vs 1.2991±0.0298).Compared with H2O2 injury group,the protein level of SP-A in dexamethasone+ H2 O2 group was down-regulated(1.4974 ± 0.0185),while SP-B,SP-C and SP-D levels were up-regulated (0.7389±0.0182,0.9244±0.0282 and 1.3339±0.0136).The differences were statistically significant respectively (all P＜0.05).Conclusions Dexamethasone may protect H441 cells from being damaged by H2O2 through regulating SP mRNA and protein expression. Key words: Oxidative stress;  Cell line, tumor;  Pulmonary surfactant-associated proteins; Dexamethasone

Key concepts: Dexamethasone, Oxidative stress, Hydrogen peroxide, Messenger RNA, Western blot, Chemistry, Pulmonary surfactant, Molecular biology

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