2002•Unpublished venueRequires access

Effect of EGF, bFGF, TGF- β1 on the proliferation of cultured chicken limbal epithelial cell

侯光辉, 江振友, 李校坤, 徐锦堂, 林晨

Abstract

目的:探讨EGF、bFGF、TGF-β1,及EGF与TGF-β1共同作用对培养鸡角膜缘上皮细胞增殖性的影响.方法:用DMEM和F12(1:1)培养基,3%胎牛血清,加入不同浓度EGF、或bFGF、或TGF-β1、或EGF与TGF-β1,采用MTT法检测培养细胞的OD值.结果:除1ng/ml组外,其余从5～160ng/ml的EGF各浓度组增殖效应均明显大于对照组(P＜0.05),10ng/ml组明显大于其它各浓度组(P＜0.02);bFGF各浓度组,除1ng/ml组和160ng/ml组外,其余从5～80ng/ml各组均明显大于对照组(P＜0.025),20ng/ml组明显大于与其它各组(P＜0.025);TGF-β1各浓度组,除0.1ng/ml组和0.2ng/ml组外,其余从0.4～12.8ng/ml各组抑制效应明显大于对照组(P＜0.025),6.4ng/ml和12.8ng/ml两组比较未见显著性差异(P=1.000),两组分别与其它各组比较均有显著性差异(P＜0.025);EGF加TGF-β1各浓度组,除1ng/ml组和160ng/ml组外,其余从5～80ng/ml各组增殖效应均明显大于对照组(P＜0.05),10ng/ml组明显大于其它各组(P＜0.025).结论:EGF、bFGF对培养鸡角膜缘上皮细胞有明显促增殖作用;TGF-β1则有明显的抑制作用;这些作用均呈剂量依赖性;EGF与TGF-β1共同表现为促增殖作用.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Transforming growth factor, Cell biology, Epidermal growth factor, Epithelium, Cell growth, Cell culture, Chemistry, Biology