Effects of resveratrol on lung injury induced by intestinal ischemia-reperfusion and activation of mast cells in lung tissues of rats

Objective To investigate the effects of resveratrol on lung injury induced by intestinal ischemia-reperfusion(I/R)and activation of mast cells in lung tissues of rats. Methods Thirty healthy female Sprague-Dawley rats, weighing 180-220 g, aged 4-5 months, were randomly divided into 3 groups(n=10 each)using a random number table: sham operation group(Sham group), intestinal I/R group(II/R group), and resveratrol group(Res group). Intestinal I/R was produced by occlusion of the superior mesenteric artery for 75 min followed by 4 h of reperfusion in anesthetized rats.In Res group, resveratrol 15 mg/kg was injected intraperitoneally once a day for 5 consecutive days before I/R.The equal volume of normal saline was given in Sham and II/R groups.All the rats were sacrificed at 4 h of reperfusion, and the lungs were removed for examination of the pathological changes which were scored and for determination of wet/dry lung weight ratio(W/D ratio), content of β-hexosaminidase(by colorimetric method), and expression of tryptase in lungs tissues(by Western blot). Results Compared with Sham group, the pathological score, W/D ratio, and β-hexosaminidase content were significantly increased, and the expression of tryptase was up-regulated in II/R and Res groups(P<0.05). Compared with II/R group, the pathological score, W/D ratio, and β-hexosaminidase content were significantly decreased, and the expression of tryptase was down-regulated in Res group(P<0.05). The pathological changes were significantly reduced in Res group as compared with II/R group. Conclusion Resveratrol can attenuate lung injury induced by intestinal I/R, and the mechanism may be related to inhibition of the activation of mast cells in lung tissues of rats. Key words: Phenols; Reperfusion injury; Intestines; Respiratory distress syndrome, adult; Mast cells